Handling and Processing of Blood Specimens from Patients with COVID-19 for Safe Studies on Cell Phenotype and Cytokine Storm.
Identifieur interne : 000598 ( 2020/Analysis ); précédent : 000597; suivant : 000599Handling and Processing of Blood Specimens from Patients with COVID-19 for Safe Studies on Cell Phenotype and Cytokine Storm.
Auteurs : Andrea Cossarizza [Italie] ; Lara Gibellini [Italie] ; Sara De Biasi [Italie] ; Domenico Lo Tartaro [Italie] ; Marco Mattioli [Italie] ; Annamaria Paolini [Italie] ; Lucia Fidanza [Italie] ; Caterina Bellinazzi [Italie] ; Rebecca Borella [Italie] ; Ivana Castaniere [Italie] ; Marianna Meschiari [Italie] ; Marco Sita [Italie] ; Gianrocco Manco [Italie] ; Enrico Clini [Italie] ; Roberta Gelmini [Italie] ; Massimo Girardis [Italie] ; Giovanni Guaraldi [Italie] ; Cristina Mussini [Italie]Source :
- Cytometry. Part A : the journal of the International Society for Analytical Cytology [ 1552-4930 ] ; 2020.
Abstract
The pandemic caused by severe acute respiratory syndrome coronavirus 2 heavily involves all those working in a laboratory. Samples from known infected patients or donors who are considered healthy can arrive, and a colleague might be asymptomatic but able to transmit the virus. Working in a clinical laboratory is posing several safety challenges. Few years ago, International Society for Advancement of Cytometry published guidelines to safely analyze and sort human samples that were revised in these days. We describe the procedures that we have been following since the first patient appeared in Italy, which have only slightly modified our standard one, being all human samples associated with risks. © 2020 International Society for Advancement of Cytometry.
DOI: 10.1002/cyto.a.24009
PubMed: 32275124
Affiliations:
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sortKey="Fidanza, Lucia" sort="Fidanza, Lucia" uniqKey="Fidanza L" first="Lucia" last="Fidanza">Lucia Fidanza</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Bellinazzi, Caterina" sort="Bellinazzi, Caterina" uniqKey="Bellinazzi C" first="Caterina" last="Bellinazzi">Caterina Bellinazzi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Borella, Rebecca" sort="Borella, Rebecca" uniqKey="Borella R" first="Rebecca" last="Borella">Rebecca Borella</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Castaniere, Ivana" sort="Castaniere, Ivana" uniqKey="Castaniere I" first="Ivana" 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last="Gibellini">Lara Gibellini</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="De Biasi, Sara" sort="De Biasi, Sara" uniqKey="De Biasi S" first="Sara" last="De Biasi">Sara De Biasi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Lo Tartaro, Domenico" sort="Lo Tartaro, Domenico" uniqKey="Lo Tartaro D" first="Domenico" last="Lo Tartaro">Domenico Lo Tartaro</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Mattioli, Marco" sort="Mattioli, Marco" uniqKey="Mattioli M" first="Marco" last="Mattioli">Marco Mattioli</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Paolini, Annamaria" sort="Paolini, Annamaria" uniqKey="Paolini A" first="Annamaria" last="Paolini">Annamaria Paolini</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Fidanza, Lucia" sort="Fidanza, Lucia" uniqKey="Fidanza L" first="Lucia" last="Fidanza">Lucia Fidanza</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Bellinazzi, Caterina" sort="Bellinazzi, Caterina" uniqKey="Bellinazzi C" first="Caterina" last="Bellinazzi">Caterina Bellinazzi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Borella, Rebecca" sort="Borella, Rebecca" uniqKey="Borella R" first="Rebecca" last="Borella">Rebecca Borella</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Medical and Surgical Sciences for Children and Adults, School of Medicine, University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Castaniere, Ivana" sort="Castaniere, Ivana" uniqKey="Castaniere I" first="Ivana" last="Castaniere">Ivana Castaniere</name><affiliation wicri:level="1"><nlm:affiliation>Respiratory Diseases Unit, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Respiratory Diseases Unit, AOU Policlinico and University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Meschiari, Marianna" sort="Meschiari, Marianna" uniqKey="Meschiari M" first="Marianna" last="Meschiari">Marianna Meschiari</name><affiliation wicri:level="1"><nlm:affiliation>Infectious Diseases Clinics, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Infectious Diseases Clinics, AOU Policlinico and University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Sita, Marco" sort="Sita, Marco" uniqKey="Sita M" first="Marco" last="Sita">Marco Sita</name><affiliation wicri:level="1"><nlm:affiliation>Department of Anesthesia and Intensive Care Unit, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Anesthesia and Intensive Care Unit, AOU Policlinico and University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Manco, Gianrocco" sort="Manco, Gianrocco" uniqKey="Manco G" first="Gianrocco" last="Manco">Gianrocco Manco</name><affiliation wicri:level="1"><nlm:affiliation>Department of Surgery, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Surgery, AOU Policlinico and University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Clini, Enrico" sort="Clini, Enrico" uniqKey="Clini E" first="Enrico" last="Clini">Enrico Clini</name><affiliation wicri:level="1"><nlm:affiliation>Respiratory Diseases Unit, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Respiratory Diseases Unit, AOU Policlinico and University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Gelmini, Roberta" sort="Gelmini, Roberta" uniqKey="Gelmini R" first="Roberta" last="Gelmini">Roberta Gelmini</name><affiliation wicri:level="1"><nlm:affiliation>Department of Surgery, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Surgery, AOU Policlinico and University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Girardis, Massimo" sort="Girardis, Massimo" uniqKey="Girardis M" first="Massimo" last="Girardis">Massimo Girardis</name><affiliation wicri:level="1"><nlm:affiliation>Department of Anesthesia and Intensive Care Unit, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Anesthesia and Intensive Care Unit, AOU Policlinico and University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Guaraldi, Giovanni" sort="Guaraldi, Giovanni" uniqKey="Guaraldi G" first="Giovanni" last="Guaraldi">Giovanni Guaraldi</name><affiliation wicri:level="1"><nlm:affiliation>Infectious Diseases Clinics, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Infectious Diseases Clinics, AOU Policlinico and University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author><author><name sortKey="Mussini, Cristina" sort="Mussini, Cristina" uniqKey="Mussini C" first="Cristina" last="Mussini">Cristina Mussini</name><affiliation wicri:level="1"><nlm:affiliation>Infectious Diseases Clinics, AOU Policlinico and University of Modena and Reggio Emilia, Modena, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Infectious Diseases Clinics, AOU Policlinico and University of Modena and Reggio Emilia, Modena</wicri:regionArea><wicri:noRegion>Modena</wicri:noRegion></affiliation></author></analytic><series><title level="j">Cytometry. Part A : the journal of the International Society for Analytical Cytology</title><idno type="eISSN">1552-4930</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The pandemic caused by severe acute respiratory syndrome coronavirus 2 heavily involves all those working in a laboratory. Samples from known infected patients or donors who are considered healthy can arrive, and a colleague might be asymptomatic but able to transmit the virus. Working in a clinical laboratory is posing several safety challenges. Few years ago, International Society for Advancement of Cytometry published guidelines to safely analyze and sort human samples that were revised in these days. We describe the procedures that we have been following since the first patient appeared in Italy, which have only slightly modified our standard one, being all human samples associated with risks. © 2020 International Society for Advancement of Cytometry.</div></front></TEI><affiliations><list><country><li>Italie</li></country></list><tree><country name="Italie"><noRegion><name sortKey="Cossarizza, Andrea" sort="Cossarizza, Andrea" uniqKey="Cossarizza A" first="Andrea" last="Cossarizza">Andrea Cossarizza</name></noRegion><name sortKey="Bellinazzi, Caterina" sort="Bellinazzi, Caterina" uniqKey="Bellinazzi C" first="Caterina" last="Bellinazzi">Caterina Bellinazzi</name><name sortKey="Borella, Rebecca" sort="Borella, Rebecca" uniqKey="Borella R" first="Rebecca" last="Borella">Rebecca Borella</name><name sortKey="Castaniere, Ivana" sort="Castaniere, Ivana" uniqKey="Castaniere I" first="Ivana" last="Castaniere">Ivana Castaniere</name><name sortKey="Clini, Enrico" sort="Clini, Enrico" uniqKey="Clini E" first="Enrico" last="Clini">Enrico Clini</name><name sortKey="De Biasi, Sara" sort="De Biasi, Sara" uniqKey="De Biasi S" first="Sara" last="De Biasi">Sara De Biasi</name><name sortKey="Fidanza, Lucia" sort="Fidanza, Lucia" uniqKey="Fidanza L" first="Lucia" last="Fidanza">Lucia Fidanza</name><name sortKey="Gelmini, Roberta" sort="Gelmini, Roberta" uniqKey="Gelmini R" first="Roberta" last="Gelmini">Roberta Gelmini</name><name sortKey="Gibellini, Lara" sort="Gibellini, Lara" uniqKey="Gibellini L" first="Lara" last="Gibellini">Lara Gibellini</name><name sortKey="Girardis, Massimo" sort="Girardis, Massimo" uniqKey="Girardis M" first="Massimo" last="Girardis">Massimo Girardis</name><name sortKey="Guaraldi, Giovanni" sort="Guaraldi, Giovanni" uniqKey="Guaraldi G" first="Giovanni" last="Guaraldi">Giovanni Guaraldi</name><name sortKey="Lo Tartaro, Domenico" sort="Lo Tartaro, Domenico" uniqKey="Lo Tartaro D" first="Domenico" last="Lo Tartaro">Domenico Lo Tartaro</name><name sortKey="Manco, Gianrocco" sort="Manco, Gianrocco" uniqKey="Manco G" first="Gianrocco" last="Manco">Gianrocco Manco</name><name sortKey="Mattioli, Marco" sort="Mattioli, Marco" uniqKey="Mattioli M" first="Marco" last="Mattioli">Marco Mattioli</name><name sortKey="Meschiari, Marianna" sort="Meschiari, Marianna" uniqKey="Meschiari M" first="Marianna" last="Meschiari">Marianna Meschiari</name><name sortKey="Mussini, Cristina" sort="Mussini, Cristina" uniqKey="Mussini C" first="Cristina" last="Mussini">Cristina Mussini</name><name sortKey="Paolini, Annamaria" sort="Paolini, Annamaria" uniqKey="Paolini A" first="Annamaria" last="Paolini">Annamaria Paolini</name><name sortKey="Sita, Marco" sort="Sita, Marco" uniqKey="Sita M" first="Marco" last="Sita">Marco Sita</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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